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10 Switching on When you switch on the unit a screen similar to the one shown here will appear briefly. This indicates the software version and b
11 5. Front panel controls The LCD Display The CYCLER TCY has a four line LCD displ
12 The keys The End, Finish or Exit key The “End” or “Finish” key is used to finish editing programs or to exit a program. It is also used to exi
13 The Down arrow key The “Down arrow” key is used for scrolling down through the lines on the screen. It is also used to decrease certain fields
14 In the prompt line is equivalent to the “End” key. On a program line means the program is locked; it can be copied and then edited but not ed
15 Programming Options There are two options available for creating programs on the CYCLER: 1. Copy and edit one of the pre-written templates. 2. C
16 A new name can now be given to the copied program (see below). To give a program a name To type in a name for the program, press "Ent
17 To edit a field or step in an existing program When the unit is switched on the display will show this screen. If known, type in the ‘Spee
18 To edit a temperature, type in the required temperature. In the example here, the heated lid temperature is being edited. Press “Enter” t
19 To copy a selected program If you select ‘Copy program’ a screen similar to this will appear and the software will automatically give the progra
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20 Example programs A couple of example thermal cycling programs are shown below. Typical 3-Step PCR Heated Lid 105 Preheat Lid on Pause
21 A new stage will be inserted which consists of a ‘Number cycles’ field and one step. Edit the number of cycles by typing the number required then
22 To add a new stage before an existing one Use the “Up Arrow” or “Down Arrow” keys to highlight the stage number. Press "En
23 To insert a ‘pause’ into a program To insert a pause after an existing stage, follow the steps for inserting a stage as above. Highlight the ‘
24 To program increment or decrement temperature or time. Under normal circumstances, the Hold Temperature of all steps is constant. However, it is po
25 The prompt line will change to flash between ‘CHANGE STEP?’ and ‘YES’ or ‘NO’. Press “Enter” The cursor will again move into the edit mode. N
26 first temperature is 65°C and the last temperature is 55°C, over 30 cycles the temperature will decrease 0.33°C each cycle. Similarly, in the examp
27 To run a program When the unit is switched on the display will show this screen. If known, type in the ‘Speed dial’ number for the requi
28 During the run the LCD screen will report: 1. The cycle number 2. The time remaining (counting down in seconds) 3. The sample temperat
29 Program Information Pressing “Enter” while a program is running will give information about the current run including which program is ac
3 Contents 1. Introduction ...4 2. Safety a
30 Access to the ‘Configuration’ set up is password protected. Default admin passwords is 1234 To enter configuration, use the alpha keys to type in
31 When you have finished, press the “Enter” key to go back to the ‘Configuration’ menu. Passwords The ‘Passwords’ function allows the admin
32 9. Notes on transferring protocols from other thermal cyclers The CYCLER TCY heats at over 3.6°C/s and provides the highest levels of temperature
33 10. User maintenance Cleaning your CYCLER The heating/cooling block, including wells and flat surfaces, should be cleaned regularly to ensure opti
34 11. Some frequently asked questions Q1 What is the ‘Pause’ function at the start of the program used for? A1 Some users prefer to preheat the h
35 12. Additional information Brief fault finding notes and a list of replacement parts are given in this section. Note that this equipment should on
WARRANTY/DISCLAIMEROMEGA ENGINEERING, INC. warrants this unit to be free of defects in materials and workmanship for aperiod of 36 months from date of
M-5380/0114Where Do I Find Everything I Need for Process Measurement and Control? OMEGA…Of Course!Shop online at omega.comSMTEMPERATUREThermocouple, R
4 1. Introduction The Cycler TCY provides the researcher with the means of accurately controlling the temperature profile of samples. It has many sci
5 Unpacking When unpacking please ensure that the following have been removed from the packaging: Thermal cycler Power cable The user is advised t
6 Working Conditions The thermal cycler is designed operate under the following conditions: Indoor use Ambient temperature range +5oC to +40oC
7 3. Cycler TCY Specification Temperature Temperature range 4°C to 99°C Temperature set point precision 0.1°C Block uniformity (over full r
8 Dimensions Height 185mm Width 185mm Length 330mm Power Power consumption 230W Units marked 100V will work at any voltage between 90 and
9 The amount of volume loss you observe and the change in reactant concentrations you can tolerate determine the minimum volume that can be used. Typi
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